Serial Two-Photon Tomography (STP tomography), a new technology developed by neuroscientists at Cold Spring Harbor Laboratory and MIT, significantly speeds up the process of acquiring highly detailed anatomical images of whole brains. Until now, the process has been painstakingly slow and available only to a handful of highly specialized research teams.
STP tomography achieves high-throughput fluorescence imaging of whole mouse brains via robotic integration of the two fundamental steps — tissue sectioning and fluorescence imaging. At 10x magnification of brain tissue samples, the researchers were able to achieve fast imaging at a resolution sufficient to visualize the distribution and morphology of green-fluorescent protein-labeled neurons, including their dendrites and axons, Osten reports.
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